Effect of labetalol on the intestinal permeability in rats with traumatic brain injury

LANG Yu-huang; CHEN Feng-yuan; XI Chen-hui

Abstract：Objective To study the effect of labetalol on tumor necrosis factor -alpha ( TNF-α) and per-meability in rats after traumatic brain injury (TBI).Methods Fifty-four male Wister rats were randomly divided into three groups:control group(n=18),TBI group(n=18) ,TBI+labetalol group(n=18).Each group was fur-ther divided into 3 hours,6 hours and 12 hours subgroups (n=6/subgroup).The rat TBI model was established by referring to Feeney ’ s method .After the model development ,animals underwent assays of vivo intestinal permeabili-ty,evaluation of plasma epinephrine and intestinal TNF-αby ELISA method at each time point .And the pathologi-cal changes of intestinal mucosa were observed under light microscope .Results The levels of plasma epinephrine , intestinal TNF-αand permeability at each time point in TBI group were significantly higher than those in control group(P<0.01).In TBI+labetalol group,the levels of plasma epinephrine and intestinal TNF-αat each time point were significantly lower than those in TBI group (P<0.01,P<0.05).At 6 and 12 hours after model establish-ment,the level of intestinal permeability in TBI +labetalol group was significantly lower than that in TBI group ( P<0.05) .Conclusion Labetalol can decrease the levels of intestinal TNF-αand lessen the increase of intestinal per-meability of rats in TBI model .