Staphylococcus aureus enterotoxin C2 induced differentiation of rabbit bone marrow mesenchymal stem cells in vitro experimental research
DONG Chen-hui
WANG Zi-ming
DU Quan-yin
WANG Yu
HUANG Kai
WANG Ai-min
Abstract：Objective To observe the induction and differentiation of Staphylococcus aureus enterotoxin C 2 (SEC2) on the growth of cultured rabbit bone marrow mesenchymal stem cells (rBMSCs) in vitro co-cultivation. Methods rBMSCs were obtained through the density gradient centrifugation and were subcultured after morphologi -cal identification.Cells were processed by applying 1,10,100,500 μM SEC2,the expression of alkaline phospha-tase (ALP) activity was detected,the cell proliferation was detected by using tetrazolium blue (MTT) assay,and os-teopontin and collagen gene expressions were detected by RT-PCR assay .Results When applying SEC 2 for induc-tion of rBMSCs,rBMSCs proliferation was inhibited with the increase of concentration (P<0.01);The co-cultivation cell proliferation with 100μM SEC2 was obviously higher than that of control group (P<0.01);16 days after induc-tion of rBMSCs with 100μM SEC2 ,mineralization and Calcium deposition were observed with osteogenesis induction detection;at the same time,on RT-PCR test,osteopontin and Collagen I were obviously increased along with the ex-tension of incubation time,indicating statistically significant differences (P<0.05).Conclusion Staphylococcus aureus enterotoxin C 2 can induce RBMSCs osteogenic differentiation ,the effect of differentiation is related to the en-terotoxin dose and the induction time .
Keywords：staphylococcal enterotoxin C 2bone marrow mesenchymal stem cellsosteogenic differentiation
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 444-448 )