Establishment of stretch injury model of in vitro cultured primary liver cells and observation on changes of their cytoskeleton and viscoelasticity
Abstract：Objective To establish the stretch injury mode l of cultured liver cells,observe the injury of liver cells and changes in th eir cytoskeleton and viscoelasticity,and explore the mechanisms.Methods After the primary liver cells were cultured in BioFlex culturing plate , they were impacted with a small biological impacting machine with pressures of 150 kPa and 250 kPa respectively. The morphological observation on changes of a rrangement and distribution of F-actin microfilament of the cytoskeleton in norm al liver cells and those with stretch injury was conducted by using a laser con- focal microscope. Meanwhile, the viscoelasticity of the liver cells was determin ed.Results After the stretch injury, most of the liver cell mic rofilaments were broken. Furthermore, their network structure disappeared. The c ells shrank to be shaped like flakes or there were only some big microfilament b ranches left. The viscoelasticity of the liver cells was significantly decreased .Conclusion The remarkable decrease of the viscoelasticity and breaking of cytoskeleton in the liver cells might be the molecular biological me chanism of changes of dynamic features in the liver cells after stretch injury.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 44-47 )