Association of DC-SIGN single nucleotide polymorphisms with severity of EV71-infected hand-foot-mouth disease
MAO Guoqi
CHEN Cen
YANG Yang
JING Wenliang
Abstract：Objective To investigate the association of dendriticcell-specific intercellular adhesion molecule-3-grabbing nonintergrin(DC-SIGN)single nucleotide polymorphisms(SNPs)with enterovirus 71(EV71)infection and disease severity in children with hand-foot-mouth disease(HFMD).Methods A total of 180 children with HFMD infected with EV71 admitted to Dazu Hospital affiliated to Chongqing Medical University during the high incidence season of HFMD(April to July and October to November each year)from 2020 to 2024 were selected as the research subjects.They were categorized into mild-to-moderate HFMD group(n=90)and severe HFMD group(n=90)according to the severity of the disease.Ninety healthy children who underwent physical examination in the hospital during the same period were included as the control group.Real-time fluorescence quantitative polymerase chain reaction was performed to determine the expression level of DC-SIGN messenger ribonucleic acid(mRNA)in peripheral blood mononuclear cells(PBMCs),and the DC-SIGN promoter region was directly sequenced.Results Compared with healthy controls,DC-SIGN mRNA levels in PBMCs in HFMD group were significantly increased(P<0.05),and the levels of DC-SIGN mRNA were significantly higher in PBMCs of the severe HFMD group compared to the mild-to-moderate HFMD group(P<0.05).The Receiver Operating Characteristic curve showed the diagnostic value of relative DC-SIGN mRNA expression levels for HFMD caused by EV71 infection and the severity of HFMD disease.After direct sequencing and comparison of DC-SIGN promoter regions from healthy controls and HFMD group only the rs7248637 GG genotype was slightly higher than that of healthy controls(P<0.05),whereas the dominant genotype GA+AA,as well as allele A,was slightly lower than that of healthy controls(P<0.05).The binary logistic model showed that the DC-SIGN rs7248637 AA genotype,and the G allele were strongly associated with the occurrence of HFMD and severe HFMD caused by EV71 infection(P<0.05).In addition,DC-SIGN mRNA levels,and the proportion of patients with severe HFMD were significantly lower compared with the GG genotype(P<0.05).Conclusion DC-SIGN SNPs are associated with susceptibility to EV71 virus and progression of HFMD disease,particularly in children carrying the rs7248637 GG genotype.The role of DC-SIGN in diagnosis of EV71-HFMD deserves further attention.
Keywords：handfoot and mouth diseasedendritic cell-specific intercellular adhesion molecule-3-grabbing nonintergrinenterovirus 71disease severitysingle nucleotide polymorphisms
Publication Date：2025-12-30
Online Publishing Date：2025-12-30(First online date of this platform, not the publication date of the document)
Pages：8( 597-604 )