Identification and analysis of clinical isolates of non-tuberculosis mycobacterium lung disease and risk factors for infection in Dalian
MAI Hongzhen
YANG Zhi
LU Xiwei
Abstract：Objective To investigate the species distribution and drug resistance profiles of nontuberculous mycobacteria(NTM)in patients with non-tuberculous mycobacteria pulmonary disease(NTM-PD)in Dalian,and to analyze host factors influencing NTM infection.Methods A screening for NTM-PD was conducted among patients admitted to Dalian Public Health Clinical Center from January 1,2023,to September 30,2024.Clinical characteristics of diagnosed NTM-PD patients were retrospectively analyzed.NTM species were identified using DNA microarray chip technology,and drug susceptibility testing(DST)was performed on major NTM species.Host factors were compared between NTM-PD and non-NTM-PD patients,and multivariate logistic regression was used to identify risk factors for NTM infection.Results A total of 200 patients were screened,with 70(35%)diagnosed with NTM-PD.The mean age of NTM-PD patients was 55.56±15.63 years,61.43%had primary infections,and 57.14%were male.The main symptoms included cough,fever,and chest tightness.Among 417 mycobacterial culture-positive isolates,83(19.90%)were identified as NTM.DST results showed high resistance rates to minocycline and sulfamethoxazole across all NTM species.Mycobacterium abscessus exhibited high resistance to rifampin,while most species showed low resistance to rifampin,azithromycin,moxifloxacin/levofloxacin,ethambutol hydrochloride,tobramycin,and cefoxitin.Multivariate logistic regression identified smoking history,diabetes,and pre-existing pulmonary diseases as significant risk factors for NTM-PD.Conclusions The predominant NTM species in Dalian were the Mycobacterium avium complex.Recommended treatment options include ethambutol,azithromycin,rifampin,moxifloxacin/levofloxacin,tobramycin,and cefoxitin.Smoking,diabetes,and underlying pulmonary diseases are key risk factors for NTM-PD.Clinicians should tailor treatment strategies based on NTM species identification and DST results to optimize patient outcomes.
Keywords：nontuberculous mycobacterial pulmonary diseasespecies identificationrisk factorshost factorsdrug resistanceinfection
Publication Date：2025-08-30
Online Publishing Date：2025-08-28(First online date of this platform, not the publication date of the document)
Pages：5( 368-372 )