HBV preS gene mutations as diagnostic biomarkers for HBV-associated hepatocellular carcinoma
CHEN Qiuyu
CHENG Zhuo
WU Yaxin
DENG Ligong
PANG Hongqua
Abstract：Objective To analyze the characteristics of hepatitis B virus(HBV)preS gene mutations in patients with hepatocellular carcinoma(HCC),and to evaluate the diagnostic value of HBV preS gene mutations in HBV-associated HCC.Methods Clinical data were retrospectively collected frompatients with chronic hepatitis B(CHB,CHB group)and HBV-associated HCC(HCC group)who admitted to the Gastroenterology,Dazhou Central Hospital,from September 2019 to September 2023.PreS mutations were analyzed by sanger sequencing.Results Patients in HCC group were older,had more genotype C,lower HBV DNA,and higher alpha-fetoprotein(AFP)level(P＜0.05).Among all patients,a total of 24.41%(31/127)patients had preS1 deletion,and 47.90%(57/119)point mutations were found in preS1 region.A total of 16.54%(21/127)patients had preS2 deletion,and 65.45%(36/55)point mutations were found in the preS2 region.PreS1 region W4P/R/Y,D27G/S,N51Y/T/S/Q/P,I/N56H/W,K57Q/K,A60V,D/A62S/T,G73S/N,A90T/S/G,V95A and L108V/I,The incidence of T125S/N/P,F141V/L/I and V158A point mutations in preS2 region was higher in HCC group than in CHB group(P＜0.05).Genotype C,HBV DNA,and W4P/R/Y(preS1),A90T/S/G(preS1),L108V/I(preS1),F141V/L/I(preS2)point mutations can independently diagnose HCC in CHB patients.Conclusion W4P/R/Y(preS1),A90T/S/G(preS1),L108V/I(preS1),F141V/L/I(preS2)in preS region are associated with HCC.The use of these point mutation tests may contribute the clinical diagnosis of HBV related HCC.
Keywords：hepatocellular carcinomahepatitis B virushepatitis B virus preSgene mutationdiagnosis
Publication Date：2025-08-30
Online Publishing Date：2025-08-28(First online date of this platform, not the publication date of the document)
Pages：7( 313-319 )