Retrospective analysis of Xpert MTB/RIF for tuberculosis diagnosis in a general hospital
LIU Zhenjia
FAN Xin
CHEN Xi
GU Li
FAN Wenping
YANG Chunxia
Abstract：Objective To investigate the application profile of the Xpert MTB/RIF at Beijing Chao-Yang Hospital,Capital Medical University,and to compare the positivity rates and concordance between Xpert MTB/RIF and acid-fast staining.Methods A retrospective analysis was conducted on clinical Xpert MTB/RIF results at Beijing Chao-Yang Hospital from 2016 to 2023.Data on departmental sources,specimen type distribution,and rpoB gene mutation characteristics of positive samples were collected.Positivity rates between Xpert MTB/RIF and acid-fast staining were compared.Results The positive rate of Xpert MTB/RIF testing at Beijing Chaoyang Hospital Affiliated to Capital Medical University from 2016 to 2023 was 3.56%,showing an overall declining trend year by year.Most positive specimens came from respiratory medicine wards,primarily lower respiratory tract samples.The detection rate of rifampicin-resistant genes was 6.35%,with Probe E mutations being the predominant type.The positivity rate of Xpert MTB/RIF was significantly higher than that of acid-fast staining(P＜0.05),with substantial agreement between the two methods(k=0.973).Conclusion Compared to acid-fast staining,Xpert MTB/RIF significantly improves the detection rate of MTB and offers superior complementary diagnostic value for the clinical diagnosis of tuberculosis.
Keywords：Xpert MTB/RIFacid-fast stainingMycobacterium tuberculosis
Publication Date：2025-06-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 221-224 )