Resistance associated protein 1,multidrug resistance associated protein 2 gene polymorphism and drug resistance in pulmonary tuberculosis
LIU Ming
ZHANG Huan
WANG Xianlei
XIE Jiong
SHEN Xuyang
Abstract：Objective To explore the clinical relationships between polymorphism of multidrug resistance associated protein 1(MRP1)and multidrug resistance associated protein 2(MRP2)gene polymorphism and drug resistance in pulmonary tuberculosis.Methods 243 Two hundred and forty-three newly diagnosed tuberculosis patients admitted to Hebei Chest Hospital from February 2021 to March 2024 were selected.Total deoxyribonucleic acid(DNA)was extracted from peripheral blood mononuclear cells,and MRP1(C218T,G2168A,G1666A locis)and MRP2(C24T,C3972T locis)gene polymorphisms were detected.Hardy-Weinberg's law was used to perform genetic balance tests on the aforementioned genes in patients.The drug resistance of pulmonary tuberculosis was detected and the patients were divided into resistant group and sensitive group,and the general information and MRP1 and MRP2 gene polymorphisms were compared between the two groups.Logistic regression analysis was used to explore the influencing factors for drug resistance in pulmonary tuberculosis.Results The genetic polymorphisms of MRP1,MRP2 in patients conformed to the Hardy-Weinberg equilibrium law(P＞0.05).The incidence of drug resistance in pulmonary tuberculosis was 21.40％(52/243).The proportions of MRP1 C218T CC,MRP1 G2168A GA,MRP1 G1666A GG,and MRP2 C24T CC genotypes in the resistant group were higher than those in the sensitive group(P＜0.05),and the allele frequencies of MRP1 C218T C,MRP1 G2168A A,MRP1 G1666A G,and MRP2 C24T C were all higher than those in the sensitive group(P＜0.05).The genotypes of MRP1 C218T CC(OR=2.016,95％CI:1.168～3.800),MRP1 G2168A GA(OR=2.155,95％CI:1.250～3.717),MRP1 G1666A GG(OR=2.168,95％CI:1.176～3.997),and MRP2 C24T CC(OR=2.252,95％CI:1.356～3.742)were all risk factors for tuberculosis drug resistance(P＜0.05).Conclusion MRP1 C218T CC,MRP1 G2168A GA,MRP1 G1666A GG,and MRP2 C24T CC genotypes can increase the risk of tuberculosis drug resistance.
Keywords：multidrug resistance associated protein 1multidrug resistance associated protein 2polymorphismPulmonary tuberculosisdrug resistance
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 26-31 )