The expression and clinical significance of serum miR-582-5p and miR-215-5p in patients with pulmonary tuberculosis
ZHU Daying
QIN Feng
Abstract：Objective To explore the expression and clinical significance of serum miR-582-5p and miR-215-5p in patients with pulmonary tuberculosis infection.Methods From March 2022 to May 2024,127 confirmed pulmonary tuberculosis patients admitted to our hospital were collected,and 96 healthy volunteers who underwent physical examinations at our hospital were selected.There was no obvious difference in age and gender between the two groups.Quantitative polymerase chain reaction(Reverse Transcription-Polymerase Chain Reaction,Reverse Transcription-Polymerase Chain Reaction,RT-PCR)was applied to detect the expression levels of serum miR-582-5p and miR-215-5p in patients.Fully automated biochemical analyzer was applied to detect serum erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),IFN-γ,and IL-12 levels.Pearson method was applied to analyze the correlation between serum miR-582-5p and miR-215-5p in patients with pulmonary tuberculosis,and their correlation with CRP,ESR,IFN-γ,and IL-12.The predictive efficacy of serum miR-582-5p and miR-215-5p expression levels in the diagnosis of pulmonary tuberculosis was analyzed using ROC curves.Results The levels of CRP,ESR,IFN-γ,and IL-12 in the tuberculosis group were obviously higher than those in the control group(P＜0.05).The expression level of serum miR-582-5p in the tuberculosis group was obviously higher than that in the control group(P＜0.05),while the serum miR-215-5p in the tuberculosis group was obviously lower than that in the control group(P＜0.05).Pearson analysis showed that the expression levels of serum miR-582-5p and miR-215-5p in patients with pulmonary tuberculosis were negatively correlated(r=-0.490,P=0.000),the expression level of serum miR-582-5p was positively correlated with CRP(r=0.415,P=0.000)、ESR(r=0.485,P=0.000)、IFN-γ(r=0.514,P=0.000)、IL-12(r=0.358,P=0.019)levels,while the expression level of serum miR-215-5p was negatively correlated with CRP(r=-0.341,P=0.021)、ESR(r=-0.391,P=0.017)、IFN-γ(r=-0.583,P=0.000)、IL-12(r=-0.416,P=0.000).levels(P＜0.05).ROC curve showed that the area under the curve(AUC)of serum miR-582-5p and miR-215-5p,and their combination in the diagnosis of pulmonary tuberculosis was 0.810,0.788,and 0.865,respectively.The combination of the 2 was superior to the individual diagnosis of serum miR-582-5p and miR-215-5p(zmiR-582-5p-combination=2.232,zmiR-215-5p-combination=2.263,P=0.026,0.024).Conclusion Detecting the expression levels of serum miR-582-5p and miR-215-5p in the body will provide new biological markers for screening patients with pulmonary tuberculosis,and can provide certain reference significance for clinical diagnosis of pulmonary tuberculosis.
Keywords：miR-582-5pmiR-215-5ppulmonary tuberculosisclinical significance
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 424-428 )