Current application of Dual RNA-seq in infectious disease research
ZHANG Lizhong
CHEN Haoran
YU Qi
LU Xuechun
Abstract：With the development of Next-generation sequencing(NGS) and the Third-generation sequencing(TGS) technology, the sequencing depth of transcriptome sequencing technology is increasing, and it is widely applicated in clinical diagnosis and treatment . Dual RNA-seq can extract the RNA of two (or more) species at the same time for interaction analysis of gene expression, and reflect the mechanism of their interaction, and can be used in medicine to analyze the interaction of the transcriptome between infected patients or experimental samples and pathogens. This allows to assess the gene expression status of patient or experimental samples and use them for drug prediction. This review summarizes the working principle of Dual RNA-seq and its application in medicine, and introduces its progress in combination with single-cell sequencing and non-coding RNA analysis, in order to provide some references for clinical and research work related to infections.
Keywords：Dual RNA-seqinfectionbioinformaticspathogenhostgene interaction regulation
Publication Date：2024-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 70-75 )