Studies on the Yinqiao powder against pseudomonas aeruginosa biofilm targeting to quorum sensing molecule
LIANG Shulan
LI Dehua
CHEN Jinwang
DENG Weihang
LIU Mingyong
Abstract：Objective To investigate the effects of Yinqiao powder on the quorum sensing molecules and biofilms of Pseudomonas aeruginosa.Methods The study used the minimum inhibitory concentration(MIC)method to detect the minimum bacteriostatic concentration of Yinqiao powder on Pseudomonas aeruginosa.The purple rod bacterial plate color reaction was used to observe the secretion of quorum sensing signal molecules by Pseudomonas aeruginosa.Gram staining was used to observe the effects of the drug on the morphology of biofilms.QRT-PCR was used to analyze the changes in gene expression of quorum sensing system and biofilm dispersal genes in Pseudomonas aeruginosa.Results The minimum bacteriostatic concentration of Yinqiao powder on Pseudomonas aeruginosa was 500 mg/ml,and Yinqiao powder had an inhibitory effect on quorum sensing signal molecules.Yinqiao powder could inhibit the formation of Pseudomonas aeruginosa biofilm;Yinqiao powder downregulated the expression levels of the quorum sensing system genes and Pseudomonas aeruginosa biofilm dispersal genes.Conclusion Yinqiao powder exerts an anti-biofilm effect on Pseudomonas aeruginosa by inhibiting the quorum sensing system.
Keywords：pseudomonas aeruginosabiofilmyinqiao powderquorum sensing
Publication Date：2023-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 410-416,422 )