To explore the value of detecting the drug-resistant Mycobacterium tuberculosis by the DNA microarray chip

HE Jian

doi:10.3969/j.issn.1007-8134.2018.01.009

Abstract：Objective To evaluate the effect of rapid test of drug-resistant Mycobacterium tuberculosis by the method of DNA microarray chip. Methods Isoniazid (INH) and rifampin (RFP) resistance were rapidly detected using DNA microarray chip method for 125 samples positive for Mycobacterium tuberculosis sputum smear and 40 samples positive for Mycobacterium tuberculosis traditional culture. At the same time, taking the traditional Mycobacterium tuberculosis drug sensitivity test as the gold standard, the consistency of drug resistance of Mycobacterium tuberculosis detected by the DNA microarray chip method was analyzed. Results The sensitivity of diagnosis of INH resistance by the DNA microarray chip method was 87.5%, and the specificity was 100%. The concordance rate with traditional drug sensitivity method was 98.8%. The sensitivity of diagnosis of RFP resistance by the DNA microarray chip method was 83.3%, and the specificity was 100%. The concordance rate with traditional drug sensitivity method was 99.4%. The sensitivity of diagnosis of multi drug resistance by the DNA microarray chip method was 100%, and the specificity was 100%. The concordance rate with traditional drug sensitivity method was 100%. Conclusions Compared with the traditional drug sensitivity method, the DNA microarray chip method has a high coincidence rate when used to detect drug resistance of Mycobacterium tuberculosis, so it has good reliability, and the detection process takes a short time. This method can be a rapid screen method on the diagnosis of tuberculosis drug resistance.