Anti-demodecidosis activity and skin safety of Jatropha curcas.l leaves extractin vitro
QIAN Jiang
CHEN Jin-shan
WEN Lei
HONG Xiao-lan
TIAN Ya-lan
Abstract：Objective To test the application value of the extract ofJatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.l leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen from demodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups ofJatropha curcas.lleaves, 2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations ofJatropha curcas.l leaves extract was determined. Skin irritation test and acute skin toxicity test were carried out in healthy rabbits, andJatropha curcas.l leaves and 75% ethanol served as experiment and control groups, respectively.Results The mite-killing time was (1.55±0.67) min with 50 mg/mlJatropha curcas.l leaves extract, (1.61±0.67) min with 25 mg/mlJatropha curcas.l leaves extract, (2.47±0.80) min with 12 mg/mlJatropha curcas.l leaves extract, and (1.20±0.48) min with 2% metronidazole. There was no significant different between 50, 25 mg/mlJatropha curcas.l leaves extract groups and 2% metronidazole group (P>0.05). The pH value was 6.07±0.73 of 50 mg/mlJatropha curcas.l leaves extract, 6.27±0.82 of 25 mg/mlJatropha curcas.lleaves extract and 6.35±0.83 of 12 mg/mlJatropha curcas.l leaves extract. Score for irritation to normal and wounded rabbit skin was both 0, and acute toxicity test showed no significant toxicity.Conclusions Jatropha curcas.l leaves extract shows a remarkable activity to demodecidosis with skin safetyin vitro.
Keywords：Jatropha curcas.l leavesdemodecidosisanti-demodecidosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 355-357 )