Ring-Box 1 gene negatively regulates anti-viral innate immune response
CHEN Xuan-nan
CHEN Qian-long
QUAN Shou-zhen
Abstract：Objective To study the role of Ring-Box 1 (RBX1/ROC1) in regulating retinoic acid-inducible gene Ⅰ (DDX58/RIG-Ⅰ) mediated anti-viral signaling pathway and explore the potential therapeutic target.Methods The full-length RBX1/ROC1 gene was amplified by PCR and inserted it in pCDNA3 plasmid. And then, the over-expression of RBX1/ROC1 in HEK293 cells was detected by Western Blot. Finally, the regulatory role of RBX1/ROC1 on DDX58/RIG-Ⅰ was detected by luciferase reporter assays.Results Flag-ROC1 eukaryotic expression plasmid was constracted. After transfected it into the HEK293 cells, Flag-ROC1 was expressed in these cells. We further found that RBX1/ROC1 downregulated DDX58/RIG-Ⅰ signaling pathway by luciferase reporter assays.Conclusions RBX1/ROC1 is a negative regulator of DDX58/RIG-Ⅰ anti-viral signaling pathway and our study provides a potential therapeutic target of anti-viral.
Keywords：RBX1/ROC1DDX58/RIG-Ⅰnegative regulated
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 91-94 )