Experiment of recombinant oncolytic influenza virus targecting effects on liver carcinoma cells
SUN Fang
REN Tian-yu
YAN Jin
DUAN Yue-qiang
WANG Xi-liang
ZHANG Shao-geng
YANG Peng-hui
Abstract：Objective To investigate the characterization and selective anti-tumor effects of the recombinant oncolytic virus rFlu△NS/HCC on liver carcinoma cells.Methods By using reverse genetics (RG), a recombinant oncolytic influenza virus rFlu△NS/HCC was rescued and identified in the backbone of A/Puerto Rico/8/34 (PR8). rFlu△NS1 based on partial deletion of NS(deNS1)was constructed, which was cotransfected with the influenza virus remaining seven plasmids of PB2, PB1, PA, HA, NP, NA and M into MDCK and COS-Ⅰcells. Recombinant rFlu△NS/HCC virus were amplified by SPF chicken embryos, purified by sucrose gradient, then morphological characteristics of virus were detected with electron microscope, hemagglutination titers and viral titers. MTS and cytopathic assay were used to investigate the oncolytic targeting effects of the recombinant rFlu△NS/HCC virus on liver carcinoma cells and normal liver cells.Results The hemagglutination titers of recombinant rFlu△NS/HCC virus was 29-10 and the log10TCID 50/ml was 6.5 of rFlu/RSV/F virus in HepG2 cells. The results were measured by MTS assay and cytopathic assay. The recombinant rFlu△NS/HCC virus could induce selective cytotoxicity in liver carcinoma cells HepG2 at MOI of 1(P<0.01), and SMMC-7721 at MOI of 3 (P<0.001), without effect in the normal liver cell line (L02). In summary, the recombinant rFlu△NS/HCC virus with differment MOI didn′t effect the normal liver cell line (L02).Conclusions The recombinant oncolytic rFlu△NS/HCC virus can induce selective cytotoxicity in liver carcinoma cells and provide a new theoretical strategy for the clinical therapy of cancer.
Keywords：influenza virusoncolytic virusliver carcinoma cells
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 86-90,103 )