Effect evaluation of MA 120 microbial identification and drug sensitivity analysis system inclinical microbiology identification and drug sensitivity test

ZHANG Ju-ling; ZHANG Cheng-long; JIA Tian-ye; WANG Huan; YUAN Yuan; PANG Jun-li; CHEN Su-ming; ZHANG Shu-yong; BAO Chun-mei; QU Fen

doi:10.3969/j.issn.1007-8134.2016.05.003

Abstract：Objective To evaluate the efficacy of identification and drug sensitivity test in clinical microbiology by MA120 microbial identification and drug sensitivity analysis system made in China. Methods A total of 1803 strains of clinical isolates from January 2013 to December 2014 of 302 Military Hospital of China, including 1205 strains of gram negative bacteria and 598 strains of gram positive bacteria, 28 genus, 107 species were collected . The strains were confirmed by 16S rDNA sequencing. The identification and drug sensitivity of bacteria were detected by MA120 and the coincidence rate of strains identification was calculated with the results of identification and sequencing. The coincidence rate of resistance rate was calculated with the results of drug sensitivity tested by MA120 and VITEK 2 automatic bacteria identification / susceptibility analysis system (VITEK 2). Results MA120 identified 1205 strains of gram-negative bacteria including19 genera and 67 species, and the coincidence rate with sequencing results was 91.12%. MA120 identified 598 strains of gram-positive bacteria including 9 genera and 49 species, and the coincidence rate with sequencing results was 94.31%. MA120 drug sensitive card covered the common antibiotics. According to the results of VITEK 2, the coincidence rate of drug resistance tested by MA120 were 89.54%(enterobacteriaceae), 86.11%(non-fermented bacteria genus), 94.74%(staphylococcus) and 82.61%(enteroptococcus and streptococcus), respectively. Conclusions M120 has a good effect in identification of common clinical microorganisms and drug sensitivity, which is used to identify bacterial species covering common clinical pathogenic bacteria and has complete drug sensitive antibiotics. As well as, it improves its application in a timely and flexible manner according to clinical requirements and recommendations of clinical and laboratory standards institue.