The influence and alteration of EpCAM expression in TACE treated hepatocellular carcinoma
LOU Min
BAI Wen-Lin
CHANG Xiu-juan
LIU Ze
LI YIN-yin
RONG Guang-hua
ZENG Zhen
Abstract：ObjectiveTo determine whether transcatheter arterial chemoembolization (TACE) treatment modulates (cancer stem cell, CSC) marker expression and the detailed function of EpCAM in HCC cells via its siRNA.MethodsThe expression levels of EpCAM were detected by qPCR in 30 HCC patients with TACE treatment and 30 HCC patients without TACE treatment, respectively. On this basis, we detected the effect to anti-tumor agents action of EpCAM in HCC cell lines HepG2 and high invasive MHCC-97H by siRNA carrier of EpCAM. Next, the siRNA of EpCAM was transfected to determine whether EpCAM modulates HCC cells’in vitro invasion and the sensitivity to anti-tumor agents.ResultsEpCAM expression levels in tumor tissues of HCC patients treated with TACE were significantly higher (P<0.05).In contrast to the control group, Transfection of EpCAM siRNA enhanced the sensitivity of HepG2 to sorafenib, oxaliplatin and epirubicin. In addition, EpCAM siRNA also disrupted the invasion of MHCC-97H cells.ConclusionsTACE can increase EpCAM expression level in HCC significantly; Reduce of EpCAM expression levels in HCC cells can lead to HCC cells of tumor stem cell characteristics such as invasion significantly decreased, and increase sensitivity to chemotherapeutic drugs.
Keywords：HCCEpCAMTACE-treatmentCSC
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 209-212,221 )