Effects of sample processing by heating at 60℃for one hour on routine clinical laboratory indicators

LI Xiao-dong; LIAO Hao; LIU Yan; LI Le; CHEN Rong-juan; LI Qi; CHEN Li; HUANG Peng-yu; WANG Xue-jing; XU Dong-ping

doi:10.3969/j.issn.1007-8134.2016.03.009

Abstract：ObjectiveTo evaluate the influence of the heating procedure on clinical laboratory indicators based on the understanding that heating at 60℃ for 1 hour is an effective assay for inactivation of Ebola virus.MethodsBlood samples of 40 subjects involved in this study were collected in sodium citrate anticoagulation tubes and EDTA-K2 anticoagulation tubes respectively, and divided into two equal aliquots. One aliquot was analyzed for biochemical, immunological, coagulation and blood routine examination parameters without preheating (prior-inactivation group), and the other was analyzed for these parameters after heating at 60℃for 1 hour (post-inactivation group). The differences in these parameters between prior-inactivation group and post-inactivation group were compared.ResultsIn terms of biochemical and immunological tests, as compared with prior-inactivation group, total protein, TBIL, CRE, TC, TG, NA, K, CL, CRP, BUN, GLU and HBsAb in post-inactivation group were not significantly different (P>0.05); ALB, GLO, PAL, ALT, AST and LDH decreased significantly, but the decreased variations were less than 80% and there was a correlation between the levels of the two groups (r>0.80,P<0.01), thus the data were still interpretable after calibration with regression equation; ALP, GGT, CHE and CK had a significant decrease (P<0.01) and no correlation was observed between the two groups. In terms of coagulation tests, D-dimer showed no significant difference between the two groups (P>0.05); the other coagulation indicators were significantly different between the two groups. In terms of blood routine tests, HGB, WBC and PLT had no significant differences between the two groups (P>0.05), provided that samples were diluted beforehand.ConclusionsHeat inactivation at 60℃ for 1 hour has no significant influence on some biochemical, immunological and blood routine tests, but significantly affects some other tests, among which 6 items are still interpretable after calibration.