Expressions of programmed death 1, programmed death ligand-1 and nerve growth factor in liver cancer tissues and their significance
YANG Shuo
ZHANG Yun-hui
SUN Yan-ling
ZHOU Zhi-ping
SHI Ming
Abstract：Objective To detect the protein expressions of programmed death 1 (PD-1), programmed death ligand-1 (PD-L1) and nerve growth factor (NGF) in hepatocellular carcinoma (HCC) tissue and investigate their relationships with different pathological classification, clinical liver cancer stages, liver function and AFP level.Methods Immunohistochemistry (IHC) was used to detect the protein expressions of PD-1, PD-L1 and NGF in the paraffin-embedded tissue specimens of 63 HCC patients. Paraffin-embedded liver tissue specimens from 16 healthy donors were used as the controls. The expressions of PD-1, PD-L1 and NGF in HCC tissues of different pathological classification, clinical liver cancer stages, liver function and AFP level were compared.Results The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens were significantly higher than that in the controls (P<0.05). The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with different pathological classification varied, being the highest in moderately differentiated HCC tissues (P<0.05). The positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with Barcelona clinical liver cancer stage C increased (P<0.05). The differences in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens with different Child-Pugh grades were not significant. The differences in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens were significant between patients with AFP level≥400μg/L and those with AFP level<400μg/L, and the rates in the former were significantly higher than those in the latter. There was no significant difference in the positive expression rates of PD-1, PD-L1 and NGF in HCC tissue specimens between patients aged over and equal to 50 and those aged less than 50.Conclusions The positive expression rates of PD-1, PD-L1 and NGF in HCC tissues increase significantly. The expressions of PD-1, PD-L1 and NGF in HCC tissues may play a role in the occurrence, development and metastasis of HCC.
Keywords：carcinomahepatocellularlymphocyte activationlymphocytesevaluation studies
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 92-96 )