Protective effect of granulocyte-colony stimulating factor on acute injury of human fetal hepatocytes

SUN Zi-jian; CHEN Jing; XIA Yan-heng; LIU Xiao-yan; SU Hai-bin; YANG Hao-zhen; XU Xiang; HU Jin-hua

doi:10.3969/j.issn.1007-8134.2016.02.007

Abstract：Objective To establish the model of acute injury of human fetal hepatocytes induced by D-galactosamine (D-GalN) and observe the protective effect of granulocyte-colony stimulating factor (G-CSF) on the injury of fetal hepatocytes. Methods The human fetal hepatocytes were hatched by gradient concentrations of D-GalN at different action time, and their activities were detected by MTT assay, in order to determine the optimal conditions for establishing the model of acute injury of human fetal hepatocytes. The human fetal hepatocytes were divided into 4 groups, which received different treatments: the first group (control group) was the blank control group; normal fetal hepatocytes of the second group (group G) were treated with G-CSF; the injury was induced by D-GalN in both the third group (group ND) and the fourth group (group GD), but the human fetal hepatocytes of group GD were treated with G-CSF, and those of group ND with the same amount of normal saline instead of G-CSF. The activities of the hepatocytes of each group were detected by both MTT assay and LDH release assay.Results More than 90% of human fetal hepatocytes could be killed by D-GalN at the concentration of 10 mg/ml for 12 hours. The medicine had enough time to play its protective effect. The activities of the hepatocytes were not significantly different between the control group and group G, while that of group GD was significantly higher than that of group ND (P<0.05).Conclusions The model of acute injury of human fetal hepatocytes can be established by D-GalN at the concentration of 10 mg/ml for 12 hours. G-CSF has a protective effect on the acute injury of human fetal hepatocytes induced by D-GalN.