Comparison of two ELISA kits for the detection of the IgG antibodies against hemorrhagic fever with renal syndrome
LI Zhuo
ZENG Han-yu
MA Ying
LIU Yuan
ZHANG Rong
LIU Mei-ning
CHEN Li-hua
Abstract：Objective To investigate the performance differences in detecting hemorrhagic fever with renal syndrome (HFRS) IgG Antibodies between domestic and imported ELISA kits. Methods Domestic and imported ELISA kits were used to detect HFRS IgG antibodies of the serum samples from 50 HFRS vaccinators, and the consistency, sensitivity, precision and methodology of the two ELISA kits were analyzed. Results Precision test showed that both the domestic and the imported ELISA kits had good precision, with the batch coefficient of variation of less than 5%. The positive consistency rate of the two ELISA kits was 100%, the negative consis-tency rate was 20%, and the similarity rate was 60%. The sensitivity of the domestic ELISA kits was 5 times higher than that of the imported ELISA kits, and the difference was significant between the two ELISA kits (P<0.05). Conclusions The sensitivity of the do-mestic ELISA kits is higher than that of the imported ELISA kits, the specificity of the imported ELISA kits is higher than that of the domestic ELISA kits, and both the domestic ELISA kits and the imported ELISA kits have good precision for the detection of HFRS IgG antibodies. The selection and application of the domestic or the imported ELISA kits depend on specific requirements.
Keywords：Hantaan virushemorrhagic fever with renal syndromeantibodiesenzyme-linked immunosorbent assay
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 92-95 )