Etiological analysis of hand, foot and mouth disease in Changzhou from 2010 to 2013

LI Jun-hong; XU Xiao-yi; WANG Feng-ming; YAO Ping; LI Qiong; XU Hui; HE Ming-zhen

Abstract：Objective To analyze the pathogens of hand, foot and mouth disease (HFMD) in Changzhou from 2010 to 2013. Methods Both rectal and throat swab samples from 1945 HFMD children in Changzhou from Jan. 2010 to Dec. 2013 were selected. The real-time fluorescence PCR was applied to detect nucleic acids of enteroviruses, and the genotype distribution according to year, age, gender and sample type, and the positive rate were analyzed statistically. Results Of 1945 HFMD patients, 591 patients (30.39%) were found positive for enterovirus (EV) 71, 526 patients (27.04%) for coxasckievirus A (CoxA) 16, and 476 patients (24.47%) for other EVs. The genotype distribution according to year was significantly different. In 2010 and 2012, the positive rate of CoxA16 was the high-est, followed by that of EV71;in 2011, the positive rate of EV71 was the highest, followed by that of CoxA16;in 2013, the positive rate of non-CoxA16/EV71 was the highest, followed by that of EV71. The total positive rate of 1~age group was higher than that of 0~age group, but the differences were not significant among the other age groups. There were significant differences in the distribution of the three genotypes among the different age groups. The positive rate of non-CoxA16/EV71 was the highest, followed by that of EV71 in 0~and 1~age groups, and the positive rate of EV71 was the highest, followed by that of and CoxA16 in 3~and 6~age groups. The positive detection rate in rectal swab group was significantly higher than that in throat swab group. Conclusions EV71 and CoxA16 are pre-dominant pathogens of HFMD in Changzhou from 2010 to 2013. HFMD mainly occurs in children at the age of 3-6 years. The positive detection rate of rectal swab samples is significantly higher than that of throat swab samples.