Evaluation of clinical diagnostic performance of interferon-gamma release assays for tuberculosis
QIN Jian-cheng
XU Jun
DAI Liang
ZHAO Jing
WANG Wei-dong
LI Xiang
ZHANG Lan
MAO Yuan-li
Abstract：Objective To evaluate the diagnostic performance and application value of enzyme-linked immunosorbent assay (ELISA)-based interferon-gamma release assays (IGRAs) with A.TB kit for patients with tuberculosis. Methods Of 108 patients en-rolled in the study, 60 were definitely diagnosed with tuberculosis (tuberculosis group), and 48 were controls (non-tuberculosis group). ELISA-based IGRAs with A.TB kit and enzyme-linked immunosorbent spot (ELISPOT)-based IGRAs with T-SPOT.TB kit were car-ried out simultaneously. Results The sensitivity and specificity of ELISA-based IGRAs with A.TB kit were 86.67% and 85.42%, and the area under a receiver-operating characteristic (ROC) curve was 0.822 [95% confidence interval (CI), 0.733-0.912]. The sensitivity and specificity of ELISPOT-based IGRAs with T-SPOT.TB kit were 91.67% and 87.50%, and the area under ROC curve was 0.884 (95%CI, 0.806-0.962). The difference in the area under ROC curve was not significant between the two assays (χ2=0.081, P=0.776). The consistency of the two assays was 87.04%(94/108) with the Kappa value of 0.738. Conclusions ELISA-based IGRAs with A.TB kit and ELISPOT-based IGRAs with T-SPOT.TB kit have a similar overall diagnostic performance, but ELISA-based IGRAs with A.TB kit is superior to ELISPOT-based IGRAs with T-SPOT.TB kit in the operation and interpretation of the test results.
Keywords：interferon-gamma release testsMycobacterium tuberculosisforecastingimmunoblottingindicators and reagents
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 302-305 )