Comparison of Bruker Biotyper system and Vitek MS system in the identification of clinical Gram-negative isolates
WANG Lu
YANG Liu
REN Wei
CHU Mei-ling
MENG Dong-ya
XUE Wen-cheng
Abstract：Objective To compare and evaluate the use of two matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) systems, Bruker MALDI Biotyper system (Bruker Biotyper system) and MALDI-TOF Vitek MS sys-tem ( Vitek MS system ) in the identification of Gram-negative clinical isolates. Methods A total of 120 Gram-negative isolates were collected from blood, urine, cerebrospinal fluid, secretion, wound swab and sputum samples from patients treated in General Hos-pital of Shenyang Military Area Command from Mar. 2012 to Jan. 2013. Each isolate was firstly identified to the species by Vitek 2 Compact system, and then identified by Bruker Biotyper system and Vitek MS system. Isolates differently identified by the three sys-tems were finally confirmed by 16S rDNA sequencing as the gold standard. Results Of the 120 isolates, 95.0%were correctly identi-fied to the genus level by Bruker Biotyper system and 92.5%by Vitek MS system, and 89.2%were correctly identified to the species level by Bruker Biotyper system and 86.7%by Vitek MS system, and the differences between them were not significant. Conclusions Bruker Bio-typer system and Vitek MS system were not significantly different in the accuracy rates of identifying clinical Gram-negative isolates. In addition, the identification results of the two systems are closely related to their own databases of pictorial works.
Keywords：spectrometrymassmatrix-Assisted laser desorption-ionizationpictorial worksGram-negative bacteria
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 282-285,295 )