Establishing a rapid two-color fluorescent PCR method for detecting the IL-28B (rs12979860) gene polymorphisms
LIU Li-ming
LI Yong-li
MA Hong-bin
WANG Xue-fei
WANG Yan
WANG Zhi-fu
MAO Yuan-li
WANG Hai-bin
Abstract：Objective To establish a rapid method suitable for routine clinical detection of interleukin (IL)-28B rs12979860 gene polymorphisms. Methods A total of 223 peripheral blood samples from hospitalized patients with hepatitis C in our hospital were randomly collected. The specific primers and fluorescent probe sequences in the sites of IL-28B rs12979860 gene polymor-phisms were designed, and IL-28B rs12979860 gene polymorphisms were detected by two-color fluorescent PCR directly, and vali-dated by gene sequencing method. The frequencies of different HCV IL-28B rs12979860 genotypes in this population were dis-cussed as well. Results The two-color fluorescent PCR technology could identify 3 genotypes of IL-28B rs12979860 single nu-cleotide polymorphisms, achieving 100%consistence with sequencing method. The frequencies of T/T, C/T and C/C genotypes in IL-28B rs12979860 gene polymorphisms were 1.79% (4/223), 23.32% (52/223) and 74.89% (167/223), respectively. Conclusions The two-color fluorescent PCR technology based on the specific probe and primer of IL-28B rs12979860 single nucleotide polymor-phisms is effective, rapid and reliable in identifying genotypes of rs12979860 gene polymorphisms. It is an effective detection method for the prediction of antiviral individualized treatment in patients with hepatitis C.
Keywords：interleukin-2hepatitis Cpolymorphismsingle nucleotidegenotype
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 279-281 )