Clinical distribution and drug resistance of pathogens causing nosocomial infection in an infectious diseases hospital in 2012

HE Wei-ping; CUI En-bo; BU Xin; BAO Chun-mei; ZHANG Wen-jin; FAN Zhen-ping; QU Fen

Abstract：Objective To investigate the distribution and drug resistance of pathogens causing nosocomial infection in an infectious diseases hospital and provide laboratory evidence for rational use of drugs in clinical practice. Methods BacT/Alert?3D automated blood culture system, blood dish and China blue agar dish were used for bacterial culture, and VITEK 2 automated identification system and API system for bacterial identification. Drug susceptibility were tested by Kirby-Bauer methods, and the results were analyzed by SPSS 13.0 statistical software. Results Escherichia coli was the most common in blood, urine and ascitic fluid samples and Pseudomonas aeruginosa was the most common in sputum samples. Antibiotic resistance of gram-negative bacteria was less than 20% to tazocillin, cefmetazole, fosfomycin and so on, and less than 10% to sulperazon, amikacin, minocycline and imipenem/cilastatin. Antibiotic resistance of gram-positive bacteria was less than 20% to amikacin, nitrofurantoin, quinupristin/dalfopristin and so on, and less than 10% to cefepime, chloramphenicol, vancomycin and teicoplanin. Vancomycin-resistant and teicoplanin-resistant Enterococcus emerged. Conclusions The common bacteria causing nosocomial infection are Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter baumannii in an infectious diseases hospital in 2012. There are great differences in antibiotic resistance of bacteria. Patients' condition, infection characteristics and drug resistance of bacteria should be taken into consideration in clinical use of drugs, so as to make individualized treatment strategies.