Inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells by M fragments of new type of reovirus
BAI Bing-ke
TAO Ling
SHEN Hong-hui
HOU Jun
HU Yan
LUO Sheng-dong
YANG Rui-chuang
MAO Pan-yong
Abstract：Objective To investigate the effects of M fragments of new type of reovirus on the inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Methods 293T cells were transfected with different recom-binant plasmids containing M1, M2 and M3 fragments respectively. Then the dual luciferase reporter system was applied to identify the effects of different genes on the inhibition of NF-κB activation. Results Three recombinant plasmids were all constructed suc-cessfully. M3 gene could inhibit tumor necrosis factor (TNF)-α-induced NF-κB activation significantly (55%reduction was detected). While M1 and M2 genes had no significant inhibitory effect on NF-κB activation. Conclusions M3 gene can inhibit TNF-α-indu-ced NF-κB activation significantly, which provides an evidence for the studies on viral pathogenesis and immunomodulatory properties.
Keywords：reoviridaeplasmidsNF-κB
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 351-354 )