Establishment of a High-quality Tomato Root Protoplast Preparation System
TIAN Honglin
LI Yanhua
LI Daozheng
ZHANG Yaolong
WANG Siting
HU Nan
Abstract：Protoplasts,which lack cell walls,hold significant potential in plant breeding,genetic transformation,and fundamental research.Enzymatic digestion is a crucial method for preparing plant protoplasts.Tomato roots are vital components of the tomato plant,and establishing a system for preparing tomato root apical meristem protoplasts preparation system is essential for understanding the intricacies of tomato root development and function.In this study,tomato(Solanum lycopersicum,cv Micro-Tom)root apices were used as the experimental material,and the concentration ratio of cellulase and pectinase was carefully optimized to develop a protocol for producing high-quality tomato root protoplasts.The results indicated that a combination of 1.5%cellulase R-10 and 1%pectinase R-10,followed by a 6 h enzymatic digestion at 25℃,yielded a protoplast concentration of 1100 cells/μl with a viability rate exceeding 90%.
Keywords：tomato rootprotoplastenzymatic hydrolysiscellulase R-10Macerozyme R-10
Publication Date：2024-11-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 107-110 )