Genetic Analysis of Nutrient Quality and Size Related Traits of Cottonseed in BC4F3∶5 Population of Cotton Chromosome Segment Introgression Lines

LUAN Yujuan; LIU Xiaofang; BAI Bingnan; QIAO Dan; LU Quanwei; GUO Jinying

doi:10.19329/j.cnki.1673-2928.2023.06.021

Abstract：In this study,the research focused on the BC4F3∶5 population of chromosome fragment substitution lines.The population was developed using Hai1 cotton as donor parent and CCRI 45 of upland cotton as the recurrent parent.The investigation and analysis of cottonseed protein content,oil content,related nutritional quality,and grain size were conducted in five different environments-Anyang,Xinjiang,Xinjiang,Zhoukou(in both 2011 and 2015).The objective was to uncover the genetic relationship between the main nutrient content traits and grain size traits in the BC4F3∶5 population of cotton chromosome segment introgression lines.The results revealed that the primary nutrients and size-related traits of cottonseed in the BC4F3∶5 population followed a normally distribution.The average values of cottonseed oil content and protein content were similar to those of the recurrent parent"CCRI 45".Moreover,the population displayed abundant genetic variation.The content of cottonseed palmitic acid showed a positive correlated with oil content.Additionally,a significant negative correlation was found between oil content and protein content.Genotype,environment and their interaction exhibited significant effects on oil content,protein content,grain width,and grain length.Notably,genotypes had a significant impact on the contents of palmitic acid,linoleic acid,and linolenic acid,while the environment significantly influenced the levels of these fatty acids.However,the interaction between genotype and environment was found to be insignificant.The findings of this research provide a theoretical foundation and serve as a reference for enhancing cotton seed quality.Furthermore,they contribute a substantial amount of foundational materials for the investigation of QTLs associated with cottonseed oil content,protein content,nutritional qualities,grainsize,and molecular breeding.