Studies on the Interaction between 2-(hydroxymethyl) Benzimidazole and DNA by UV Spectrophotometry
YU Youzhu
SONG Qunli
GUO Yuhua
Abstract：Purpose:the interaction between 2-(hydroxymethyl)benzimidazole and DNA was studied by UV spec?trophotometry and viscosity measurements, and influence of ion strength on the binding of 2-(hydroxymethyl) benzimidazole with DNA was also investigated. Results:DNA resulted in some decrease and red shift in the ab?sorption of 2-(hydroxymethyl)benzimidazole. The binding constant for 2-(hydroxymethyl)benzimidazole with DNA was 5.2 × 107 L?mol-1. The relative viscosity of DNA increased in the presence of 2-(hydroxymeth yl)benz?imidazole, there was no influence of ion strength on the absorbance of 2-(hydroxymethyl)benzimidazole-DNA. Conclusion:the binding of 2-(hydroxymethyl)benzimidazole with DNA was an intercalation binding.
Keywords：2-(hydroxymethyl)benzimidazoleDNAUV spectrophotometryviscosityinteraction
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-11,21 )