No DCX-positive neurogenesis in the cerebral cortex of the adult primate
Ruo-Xu Liu1
Jie Ma1
Bin Wang1
Tian Tian2
Ning Guo1
Shao-Jun Liu1
1.Institute of Military Cognition and Brain Sciences, Beijing, China2.Department of Pharmacy, Medical College, Huanghe S&T University, Zhengzhou, Henan Province, China
摘要：Whether endogenous neurogenesis occurs in the adult cortex remains controversial. An increasing number of reports suggest that double-cortin (DCX)-positive neurogenesis persists in the adult primate cortex, attracting enormous attention worldwide. In this study, different DCX antibodies were used together with NeuN antibodies in immunohistochemistry and western blot assays using adjacent cortical sec-tions from adult monkeys. Antibody adsorption, antigen binding, primary antibody omission and antibody-free experiments were used to assess specificity of the signals. We found either strong fluorescent signals, medium-weak intensity signals in some cells, weak signals in a few perikarya or near complete lack of labeling in adjacent cortical sections incubated with the various DCX antibodies. The putative DCX-positive cells in the cortex were also positive for NeuN, a specific marker of mature neurons. However, further experiments showed that most of these signals were either the result of antibody cross reactivity, the non-specificity of secondary antibodies or tissue autoflu-orescence. No confirmed DCX-positive cells were detected in the adult macaque cortex by immunofluorescence. Our findings show that DCX-positive neurogenesis does not occur in the cerebral cortex of adult primates, and that false-positive signals (artefacts) are caused by antibody cross reactivity and autofluorescence. The experimental protocols were approved by the Institutional Animal Care and Use Com-mittee of the Institute of Neuroscience, Beijing, China (approval No. IACUC-AMMS-2014-501).
机标关键词：
分类号：R453(治疗学)R363(病理学)R364(病理学)
论文发表日期：2020-07-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1290-1299 )
英文信息