摘要：Objective: To investigate the relationship between gene mutation and pathological type of lung cancer, inspect and verify the consistency between homologous genes mutation in various pathologic type. Methods: Combined with the COSMIC and UniProt database, we obtained the reported overall big-sample mutation data of lung cancer and the protein sequences of the top 20 mutated genes, respectively. Analyze the data and cluster the protein sequences and then deduce the homologous gene. Ultimately, analyze the mutations of different pathological types of homologous genes. Results: TP53 (32.32％) has the highest mutation rate in lung cancer, followed by EGFR (29.12％). The copy number variability (CNV) of genes: KRAS, LRP1B, CDKN2A, KMT2C, FAT1, PIK3CA, RB1, ERBB4, GRIN2A and KDR between each pathological type is statically significant (P<0.05). The gene differential expression rate between adenocarcinoma and squamous carcinoma of gene TP53, KRAS, LRP1B, CDKN2A, STK11, FAT4, KMT2D, NFE2L2, KEAP1, PIK3CA, RB1, ERBB4, SMARCA4 and KDR are statistically significant (P<0.05). The similarity of the protein sequence of EGFR and ERBB4 can reach 93％, and FAT4 and FAT1 are 81％. For small cell carcinoma, there's no difference in CNV between the two groups of homologous genes, and no difference between FAT4 and FAT1 in adenocarcinoma. Conclusion: The CNV and gene expression of lung cancer-associated genes are relevant to pathologic types. GFR and ERBB4 are homologous, FAT4 and FAT1 are also among the top 20 mutation genes. Additionally, there's no difference in CNV between the two groups of small cell carcinoma, which is the same between FAT4 and FAT1 in adenocarcinoma.

摘要： Ballistocardiogram signal monitoring system based on GSM network was put forward in this paper. The system included a BCG signal acquisition module, a data processing module, a display module and a GSM module. The STM32F103VB microprocessor was used as the controlling core of the signal acquisition module. BCG signal acquisition, amplification, filtering and A/D conversion were completed by the resistance strain sensor and high precision A/D conversion chip of TM7708; VB6.0 software was used to realize the BCG signal analysis and processing;the SD card and LCD completed data storage and waveform display; the BCG data remote transmission and alarm function were realized through the GSM module. The system cannot only real-time monitor the changes of heart rate of patients by non-contact means, and can process data automatically, timely detection of arrhythmia and automatic alarm. The system is particularly suitable for heart disease patients receiving long-term home care;therefore, it has a broad application prospect.

摘要：Intraventricular hemorrhage (IVH) is one of the most severe medical emergencies in neurosurgery. Early detection or diagnosis would significantly reduce the rate of disability and mortality, and improve the prognosis of the patients. Although present medical imaging techniques have high sensitivity to identify bleeding, the use of an additional, non-invasive imaging technique capable of continuously monitoring IVH is required to prevent contingent bleeding or re-bleeding. In this study, electrical impedance tomography (EIT) was applied to detect the onset of IVH modeled on 6 piglets in real time, with the subsequent process being monitored continuously. The experimental IVH model was introduced by one-time injection of 2 ml fresh autologous arterial blood into the ventricles of piglets. Results showed that resistivity variations within the brain caused by the added blood could be detected and imaged in vivo using the EIT method, and the magnitude and the size of region of interest on EIT images may be linearly associated with the volume of the blood. In conclusion, EIT has unique potential for use in clinical practice to provide invaluable real-time neuroimaging data for IVH after the improvement of electrode design, anisotropic realistic modeling, and instrumentation.

摘要： This paper aims to the research of the impact of fluid shear stress on the adhesion between vascular endothelial cells and leukocyte induced by tumor necrosis factor-α(TNF-α) by microfliudic chip technology. Microfluidic chip was fabricated by soft lithograph;Endothelial microfluidic chip was constructed by optimizing types of the extracellular matrix proteins modified in the microchannel and cell incubation time;human umbilical vein endothelial cells EA.Hy926 lined in the microchannel were exposed to fluid shear stress of 1.68 dynes/cm2 and 8.4 dynes/cm2 respectively. Meanwhile, adhesion between EA.Hy926 cells and leukocyte was induced by TNF-αunder a flow condition. EA. Hy926 cell cultured in the static condition was used as control group. The numbers of fluorescently-labeled leukocyte in microchannel were counted to quantize the adhesion level between EA. Hy926 cells and leukocyte; cell immunofluorescence technique was used to detect the intercellular adhesion molecule (ICAM-1) expression. The constructed endothelial microfluidic chip can afford to the fluid shear stress and respond to exogenous stimulus of TNF-α;compared with the adhesion numbers of leukocyte in control group, adhesion between EA. Hy926 cells exposed to low fluid shear stress and leukocyte was reduced under the stimulus of TNF-α at a concentration of 10 ng/ml(P<0.05);leukocyte adhesion with EA. Hy926 cells exposed to high fluid shear stress was reduced significantly than EA. Hy926 cells in control group and EA.1Hy926 cells exposed to low fluid shear stress ( P<0.01); the regulation mechanism of fluid shear stress to the adhesion between EA. Hy926 cells and leukocyte induced by TNF-αwas through the way of ICAM-1. The endothelial microfluidic chip fabricated in this paper could be used to study the functions of endothelial cell in vitro and provide a new technical platform for exploring the pathophysiology of the related cardiovascular system diseases under a flow environment.

摘要：This paper presents the analysis and management system of digital ultrasonic image. The system can manage medical ultrasonic image by collecting, saving and transferring, and realize that section offices of ultrasonic image in hospital network manage. The system use network technology in transferring image between ultrasonic equipments to share patient data in ultrasonic equipments. And doctors can input patient diagnostic report,saved by text file and case history, digitally managed. The system can be realized by Visual C++ which make windows applied. The system can be brought forward because PACS prevail with various hospitals,but PACS is expensive. In view of this status, we put forward to the analysis and management system of digital ultrasonic image,which is similar to PACS.

摘要： Calcium phosphate ceramics are widely used as biomaterials to substitute or/and repair hard tissues because of their excellent biocompatibility and bioactivity. Among them, hydroxyapatite (HA) is the most promising material. However, their mechanical brittleness restrains them from being used as load-bearing implants.

摘要： Cell affinity is an important factor to be concerned when biodegradable polymeric materials are utilized as cell scaffold in tissue engineering . Many studies haveproved that hydrophilicity , surface energy, charge and roughness of the materialsurface greatly influence the cell attachment and cell growth on the material.

摘要：Objective: To make statistics on the distribution of ApoE gene polymorphism in the Longyan area, provide data for the statistics of regional cerebrovascular diseases, explore the significance of ApoE gene polymorphism distribution for clinical guidance, diagnosis and treatment, and provide more suggestions for clinical diagnosis and treatment of cerebrovascular diseases. Methods: 800 patients with cerebrovascular disease in the Longyan area from July 2018 to April 2021 were selected as the research object,and 800 normal healthy patients were selected as the control group. The data were further analyzed according to the collected patient information, and the patients were distinguished according to age and other basic information, the type of cerebrovascular disease, ApoE gene polymorphism and blood lipid test results. The distribution of ApoE gene polymorphism in the cerebrovascular disease population in the Longyan area was given to determine the correlation between the incidence of cerebrovascular disease and the distribution of ApoE gene polymorphism. Results:In the study group, E2/E3 genotyping was the most common 400 (50.00％), and E4/E4 genotyping 6 (0.75％) was the least common;In the control group, E2/E4 genotyping was the most common 480(60.00％), and E2/E3 genotyping accounted for the second 290 (36.25％). In the study group, E3 allele was the most common 383 (47.88％),E4 allele 121 (15.12％);in the control group, E2 allele was the most common 391 (48.88％), and E3 allele 142 (17.75％). Conclusion: According to the statistics of ApoE gene polymorphism distribution in the Longyan area and the analysis of the correlation of other cerebrovascular diseases,it is found that E2/E3 genotype may be related to cerebrovascular diseases, and E3 allele may be a susceptibility factor. Through the above analysis, the occurrence of diseases can be predicted and prevented.

摘要：A frequency following response (FFR) of speech auditory brainstem response (speech-ABR) elicited by the speech syllable/da/contains three distinct waves named as D, E and F, corresponding to the structure of the stimulus sound. The detection and characterization of FFRs are critical in the study and application of speech-ABRs. Conventional methods detect the latencies of the waves in time domain by measuring the maximal amplitudes of the waveform in the preset windows, which suffers the problem of low quality of FFR waves. In this paper, we defined an instantaneous energy (IE) spectrum based on empirical mode decomposition (EMD) method (EMD-IE method) to detect FFR and measured the latencies of the waves. The results reveal that the FFRs are mostly evident on the second layer of the IE spectra, which would benefit the detection and measurement of the FFRs in clinic.

摘要：The recognition of electroencephalogram (EEG) signals is the key of brain computer interface (BCI).Aimed at the problem that the recognition rate of EEG by using support vector machine (SVM) is low in BCI,based on the assumption that a well-defined physiological signal which also has a smooth form"hides" inside the noisy EEG signal,a Quasi-Newton-SVM recognition method based on Quasi-Newton method and SVM algorithm was presented.Firstly,the EEG signals were preprocessed by Quasi-Newton method and got the signals which were fit for SVM.Secondly,the preprocessed signals were classified by SVM method.The present simulation results indicated the Quasi-Newton-SVM approach improved the recognition rate compared with using SVM method; we also discussed the relationship between the artificial smooth signals and the classification errors.

摘要：Objective: To explore the diagnostic value of tb-RNA in pleural effusion in tuberculous pleurisy. Methods: 60 patients with tuberculous pleurisy treated from March 2018 to September 2019 were selected as the research object, and 60 patients with non-tuberculous pleurisy treated at the same time were selected as the control group to analyze the diagnostic consistency of tuberculous pleurisy. Results: Through the consistency analysis of the gold standard and tb-RNA diagnosis, the diagnosis consistency of the two methods was strong. By comparing the tb-RNA level of the two groups, the tb-RNA level of the observation group was significantly higher than that of the control group (P<0.05); Through the analysis of diagnostic efficacy of tb-RNA, the diagnostic sensitivity and specificity of tb-RNA were 58.33％and 97.50％, respectively. Conclusion: tb-RNA detection of pleural effusion has a high positive diagnostic value and short detection cycle, which is of positive significance for the diagnosis of tuberculous pleural effusion.

摘要：This paper presents a novel method of spot addressing and segmentation about the foreground segmentation of microarray image.In this paper,a spot addressing method based on particle swarm optimization (PS O),algorithm is proposed to have a further search for the center coordinate and radius of the spot whose region is determined by the projection method.Then,a foreground segmentation method is put forward to make the spot foreground segmentation based on the center coordinate and radius of the spot.The spot addressing and segmentation experiments on synthetic and real microarray images show that the proposed method is effective and feasible for the foreground segmentation of microarray image.

摘要： One of the challenges in the field of tissue engineering is the development of biomaterial/cell interactions. For this purpose, the use of biological materials in tissue engineering requires the application of different chemical or physical procedures for surface modification in order to improve the cell affinity of biomaterials. We have studied the effects of one chemical treatment and one physical treatment: 1-(3-dimethylaminopropyl )-3-ethylcarbodiimide (WSC) and entrapment.

摘要： Glioma has the highest incidence in the brain tumors. Though treated with surgical resection, external beam radiation therapy, and systemic chemotherapy, patients with glioma have poor prognosis because of glioma recurrence.

摘要：In biology ferment engineering, accurate statistics of the quantity of bacte-ria is one of the most important subjects. In this paper, the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and pro-cessing system is designed to accomplish image preprocessing, image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully real-ized by means of a region-based level set method and then the quantity of bacteria is com-puted precisely, which plays an important role in optimizing the growth conditions of bac-teria.

摘要：Developing a shear thinning non-Newtonian fluid to substitute blood is desirable in studies of biomedicine engineering since blood is a shear thinning nonNewtonian fluid that exhibits both viscous and elastic properties, and generally not available in large volume. The rheological properties of aqueous Xanthan gum with different concentrations and temperatures were studied in present paper. The results show that aqueous Xanthan gum is a non-Newtonian fluid which displays remarkable shear thinning behaviour and is a suitable blood analog fluid. There is a power law distribution relationship between shear stress and shear rate, and the higher the solution concentration is, the more strongly it displays shear thinning behaviours. Viscosity values of aqueous Xanthan gum increase with the solution temperature decrease or with the solution concentration increase in linearity. Moreover at the temperature of 37℃, aqueous Xanthan gum with concentration of 0.4‰ and 0.6‰ matches human blood best in rheological properties. According to the resuits, the viscosity expression varied with temperature and concentrition is obtained, and the stability of Xanthan gum solution is discussed.