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["Bigmouth Bass Major Diseases and Prevention Measures (II)"]
Abstract:["(4) Nucleic acid detection. Extract DNA from diseased fish tissues, select the upstream primer gyrBF1: 5\u0027-GCATGGAGACCTTCAGCAAT-3\u0027 and the downstream primer gyrBR1: 5\u0027-GCGGAGATTTTGCTCTTCTT-3\u0027, perform PCR amplification, the target fragment size is 415 base pairs. Then perform agarose gel electrophoresis, according to whether the target band exists in the electrophoresis results, it can be determined, and nucleotide sequence alignment can also be used to identify the bacterial species."]
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Publication Date:2025-10-10
Online Publishing Date:2025-11-06(First online date of this platform, not the publication date of the document)
Pages:3( 1-3 )
Scientific Fish Farming

Scientific Fish Farming

ISSN:1004-843X
Year, Vol.(Issue):2025,(10)