Screening of Toxinogenic Medium for Hulless Barley Sheath Rot and Study on Phytotoxicity of Fermentation Products
DONG Ruifang
CHEN Liyifan
LIU Yan
ZHEN Haowen
ZHANG Haifeng
YAO Qiang
Abstract：In recent years,the occurrence range of highland barley sheath rot in China has gradually expanded,and the degree of harm has increased year by year.The fungal disease has gradually risen to become the main disease in highland barley production areas,which has brought serious challenges to the safe production of highland barley.In this study,the phytotoxicity of the secondary metabolites of Dactylobotrys graminicola was studied.Through the comparison of the phytotoxicity of fermentation products in different media,it was found that the fermentation products of oligotrophic culture broth had the strongest phytotoxicity,which significantly inhibited the germination of barley seeds(germination rate was 0)and the growth of roots and shoots(the inhibition rate of root length and shoot length was 100％).By means of Scanning electron microscopy(SEM)and transmission electron microscopy(TEM),it was observed that phytotoxic metabolites could significantly damage host cell structure.Through the monitoring of the enzyme activity of the host plant,it was found that the fermentation products with plant toxicity could inhibit the CAT and POD activities of the host plant.The results of this study can lay a foundation for the subsequent revelation of the pathogenic mechanism of hulless barley sheath rot.
Keywords：Hulless barley sheath rotSecondary metabolitePhytotoxicityBioassay
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 51-59 )