Optimization of Mature Embryo Culture System of Naked Oat and Preliminary Study on Transformation System of Traumatized Embryo
MA Jiaxing
LI Qinghua
LIU Yuhai
NAN Jinsheng
RONG Xiaoping
LU Suxiang
HAN Bing
Abstract：Callus induction and differentiation culture in the mature embryos of 8 naked oat varieties were performed.Naked Oat No.1 was selected as the variety with the highest healing rate,and the medium was further optimized.The results showed that No.1 oat had the healing rate of 87.33％in ML2 induction medium and higher differentiation rate in DM1 differentiation medium.The optimal culture system was as follows:ML2 induction medium was used to induce callus,subculture was carried out after about 15 days,followed by two subcultures and then transferred to DM1 differentiation medium,and then transferred to rooting medium after differentiation of leaves.The pBI121 plasmid with GFP gene was used to infect the callus embryos of naked Oat No.l by Agrobacterium-mediated method,and the effects of concentration of bacterial solution,concentration of selective agent and transplanting time on the transformation rate were investigated.The results showed that the efficient combined culture system for callus embryo transformation of naked oats included Agrobacterium solution(OD600)of 0.8 for infection,the optimal concentration of kanamycin selective agent was 40 mg/L,and the callus embryo was transplanted after 10 days of transformation culture.The Agrobacterium-mediated genetic transformation system of callus embryos of naked oats preliminarily established in this experiment is feasible,which provides a reference for the establishment of the regeneration system of naked oats in the future.
Keywords：Naked oatTissue cultureGenetic transformationAgrobacterium-mediated transformation
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 21-28,36 )