Construction and Analysis of the SSH Libraries of the Tibetan Hulless BarleyVariety Zangqin 13 Treated with High Nitrogen
XU Qi-jun
WANG Yu-lin
YUAN Hong-jun
ZENG Xing-quan
NIMA Zha-Xi
Abstract：To analyze the genes responsive to high -nitrogen treatment in Tibetan hulless barley,suppression subtractive hybridization (SSH)was utilized to construct the differential expression cDNA libraries using the hulless barley variety Zangqin 13.From the two constructed cDNA libraries,281 positive clones were randomly selected and subjected to sequencing;consequently,219 qualified ESTs containing 212 unigenes were obtained.The similarity analysis of expressed sequence tags (ESTs)of these unigenes was performed with Blastx and Blastn against the non -redundance database in GenBank.The results showed that the 212 sequences were homologous with some genes or proteins in GenBank,which are responsible for biological process,cellular components,and molecular functions according to the analysis of Blast2Go.In the analysis of KEGG,117 ESTs of the 212 sequences were mapped to 17 metabolic path-ways.Specifically,these unigenes encode enzymes that are involved in the structure and function of some metabolic synthesis path-ways;as well as in signal transduction,transcriptional regulation,and photosynthesis.This suggests that these genes may contribute to the tolerance of Tibetan hulless barley to high nitrogen treatment.
Keywords：Tibetan hulless barleyHigh nitrogen treatmentSuppression subtractive hybridization (SSH)Expressed sequence tags (ESTs )
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )