Screening Barley Varieties with High Regeneration Rate Using Mature Embryo Culture
Abstract：In order to screen barley varieties for genetic transformation, mature embryo culture efficiency of 30 barley varieties was compared using the modified culture medium in this paper. The results showed the callus induction rate of mature embryo was significant difference in 30 barley varieties. Morex callus induction rate reached 94.95% , short awn purple barley callus induction rate was less than 6%. Compared with the whole embryo, the broken emblTo can be obtained relatively good callus induction effect and lower germination rate, time. The differ- entiation rate was also significant difference in 30barley varieties, zhexiu22 differentiation rate reached 97.6% , zhudamai5 differentiation rate is lower, only 11.85%. In the regeneration rate, different varieties have significant difference, Golden promise regeneration rate is higher, 44.7% , kenpimai9 regeneration rate is relatively low, only 1.4%. Yangnongpi9 albino occurrence rate is higher, reaching 13.25% , 23 varieties of albino occurrence rate is less than 10%. Comprehensive analys good culture effect and higher transformation recipient. is, Golden promise, Zhepi8, Zhexiul2, Metcalfe, Franklin, Zheda8 have of mature embryo regeneration ability, they can be used as barley genetic
Keywords：BarleyVarietyMature EmbryoTissue Culture
Publication Date：2012-04-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )